Contact an Account ManagerSpecific simultaneous assay of hypoxanthine and xanthine by reversed phase HPLC using an immobilized xanthine oxidase reactor
Summary
Type: HPLC
Application #: 107070
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: Fig. 7: UV, wavelengths as indicated
Fog. 3: fluorescence, excitation 320nm, emission 407nm
Fig. 2: UV, 290nm
Conditions: Fig. 7: eluent: 0.01M K phosphate pH 4.8, flow rate 0.7 ml/min; effluent from the column mixed with 0.01M sodium borate pH 9.2, flow rate 0.23ml/min to adjust the pH to 7.4
Fog. 3: eluent: 0.0M phosphate buffer pH 5.5 containing 2% acetonitrile, flow rate 0.7ml/min; effluent from the column adjusted to pH 7.5 with 0.1M phosphate buffer pH 10, flow rate 0.34ml/min; effluent from xanthine oxidase reactor adjusted to pH 8.0 with 0.1M phosphate buffer pH 10, 0.45ml/min
Fig. 2: eluent: 0.01M phosphate buffer pH 5.5 containing 1% acetonitrile, flow rate 0.7ml/min; flow rate of 0.01M sodium borate 0.27ml/min
Authors: Tawa R
Source: Chem Pharm Bull 30 (1982) 615 - 621
Details
Legend: Fig. 7. HPLC profiles of a mixture of hypoxanthine, xanthine and uric acid (A,
B and C), and with the use of reactor II (D), 1. unknown, 2. uric acid, 3.
hypoxanthine, 4. xanthine.
Fig. 3. Chromato